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Complementary Fe3+- and Ti4+-immobilized metal ion affinity chromatography for purification of acidic and basic phosphopeptides

✍ Scribed by Alan Chuan-Ying Lai; Chia-Feng Tsai; Chuan-Chih Hsu; Yu-Ni Sun; Yu-Ju Chen


Book ID
115561839
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
743 KB
Volume
26
Category
Article
ISSN
0951-4198

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## Abstract The selective enrichment of phosphopeptides with good reproducibility is vital for the in‐depth study of the phosphoproteome. Herein, we presented a novel method to prepare monolithic Ti^4+^ or Zr^4+^ immobilized metal affinity chromatography (IMAC) columns. Since succinimide was of hig