Complementary Fe3+- and Ti4+-immobilized metal ion affinity chromatography for purification of acidic and basic phosphopeptides
β Scribed by Alan Chuan-Ying Lai; Chia-Feng Tsai; Chuan-Chih Hsu; Yu-Ni Sun; Yu-Ju Chen
- Book ID
- 115561839
- Publisher
- John Wiley and Sons
- Year
- 2012
- Tongue
- English
- Weight
- 743 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0951-4198
- DOI
- 10.1002/rcm.6327
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## Abstract The selective enrichment of phosphopeptides with good reproducibility is vital for the inβdepth study of the phosphoproteome. Herein, we presented a novel method to prepare monolithic Ti^4+^ or Zr^4+^ immobilized metal affinity chromatography (IMAC) columns. Since succinimide was of hig