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Competitive reverse transcription polymerase chain reaction for quantifying pre-MRNA and mRNA of major acute phase proteins

✍ Scribed by Liselotte E. Jensen; Alexander S. Whitehead

Book ID: 114052013
Publisher: Elsevier Science
Year: 1998
Tongue: English
Weight: 352 KB
Volume: 215
Category: Article
ISSN: 0022-1759
DOI: 10.1016/s0022-1759(98)00085-4

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## Abstract There are several methods available to analyze the mRNA concentration quantitatively. Among them, the competitive reverse transcription (RT‐)PCR method is very useful. For this method, Cy5‐labeled primers were used, and after gel electrophoresis in 7 M urea, the Cy5‐labeled single‐stran