## Abstract There are several methods available to analyze the mRNA concentration quantitatively. Among them, the competitive reverse transcription (RT‐)PCR method is very useful. For this method, Cy5‐labeled primers were used, and after gel electrophoresis in 7 M urea, the Cy5‐labeled single‐stran
✦ LIBER ✦
Competitive Reverse Transcription–Polymerase Chain Reaction for Determination of Rat CC and C Chemokine mRNAs
✍ Scribed by Zhou Luo Ou; Yumiko Natori; Naoko Doi; Kiyoshi Kawasaki; Masahiro Nishijima; Yasuhiro Natori
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 153 KB
- Volume
- 261
- Category
- Article
- ISSN
- 0003-2697
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## Abstract Increasing evidence suggests that the chemokine interleukin (IL)‐8/CXCL8 plays important roles in CNS development, neuronal survival, modulation of excitability, and neuroimmune response. Recently, we have shown that CXCL8 can acutely modulate ion channel activity in septal neurons expr