Competitive enzyme-immunoassays using native viral antigens to discriminate between HIV-1 and HIV-2 infections
✍ Scribed by Armelle Baillou; Francis Barin; Guy Léonard; Francois Denis; Eric Petat; Wanda F. Canas Ferreira; Souleymane M'Boup; Alain Goudeau
- Book ID
- 119114833
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 720 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0166-0934
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For diagnosis of HIV-1 infection, attempts were made to detect anti-HIV-1 IgG in urine by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) US- ing recombinant reverse transcriptase (RT) and p17 as antigens. Anti-HIV-1 IgG in urine was reacted simultaneously with 2,4-dinitrop
Anti-HIV-1 IgG in urine was detected by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant reverse transcriptase (RT), p i 7 and p24 as antigens, and p-o-galactosidase from Escherichia coli as label. Anti-HIV-I IgG in urine was reacted simultaneously
Recombinant p17 (rp17) antigen of HIV-1 and maltose binding protein-rp17 fusion protein (MBP-rp17) were immobilized onto polystyrene beads in different ways: rp17 and MBP-rp17 were immobilized directly onto polystyrene beads by physical adsorption; biotinyl-rp17 and biotinyl-MBP-rp17 were immobilize
Anti-HIV-1 IgG in urine was detected by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant p24gagprotein (p24) of HIV-1 as antigen and P-D-galactosidase from fscherichia colias IabeLAnti-HIV-1 IgG in urine was reacted simultaneously with 2,4dinitrophe
Whole saliva samples collected from HIV-1 seropositive subjects by simple spitting without using any devices were dried on filter paper strips, from which filter paper discs of 3-mm diameter were punched out. The eluates of the discs were subjected to the immune complex transfer enzyme immunoassay f