Whole saliva dried on filter paper for diagnosis of HIV-1 infection by detection of antibody IgG to HIV-1 with ultrasensitive enzyme immunoassay using recombinant reverse transcriptase as antigen

✍ Scribed by Setsuko Ishikawa; Seiichi Hashida; Kazuya Hashinaka; Kouichi Hirota; Masaaki Kojima; Atsushi Saito; Akihisa Takamizawa; Hideo Shinagawa; Shinichi Oka; Kaoru Shimada; Eiji Ishikawa

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 478 KB
• Volume: 10
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/(sici)1098-2825(1996)10:1<35::aid-jcla6>3.0.co;2-k

✦ Synopsis

Whole saliva samples collected from HIV-1 seropositive subjects by simple spitting without using any devices were dried on filter paper strips, from which filter paper discs of 3-mm diameter were punched out. The eluates of the discs were subjected to the immune complex transfer enzyme immunoassay for antibody IgG to HIV-1 using recombinant reverse transcriptase of HIV-1 as antigen and a two-site enzyme immunoassay for whole IgG. The signals for antibody IgG to HIV-1 and the amounts of whole IgG obtained with one disc per assay tube were 126-290% of those obtained with 1 pI of whole saliva samples, providedthat filter paper strips were treated with nonspecific rabbit serum prior to drying whole saliva samples and that filter paper discs were tested within a few days after drying whole saliva samples. From these results, diagnosis of HIV-1 infection was indicated to be possible with whole saliva samples dried on filter papers, since the diagnosis was previously shown to be possible with 1 bI of whole saliva samples. The test for HIV-1 infection with whole saliva samples dried on filter papers was suggested to be useful for various purposes.