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Competitive binding assay for glucocorticoids. Influence of experimental conditions on measurement of the affinity of competitive steroids for the receptor

✍ Scribed by Alessandro Weisz; T.William Hutchens; Francis S. Markland Jr.


Book ID
116002550
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
480 KB
Volume
16
Category
Article
ISSN
0022-4731

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Competitive binding assays for high-affi
✍ Richard W. Schreiber Jr.; Michael A. Letavic; Timothy J. McGahan; Harold B. Whit πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 731 KB

Endogenous ligands complicate radioligand-binding assays of high-affinity binding proteins by obscuring binding sites or by diluting the labeled ligand. We have developed a mathematical model for such systems where radioligand and endogenous ligand are structurally identical. Data which relate radio