✦ LIBER ✦

Compensation for Loss of Ligand Activity in Surface Plasmon Resonance Experiments

✍ Scribed by Raimund J. Ober; E.Sally Ward

Publisher: Elsevier Science
Year: 2002
Tongue: English
Weight: 132 KB
Volume: 306
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.2002.5716

No coin nor oath required. For personal study only.

✦ Synopsis

The determination of equilibrium binding constants is an important aspect of the analysis of protein-protein interactions. In recent years surface plasmon resonance experiments (e.g., with a BIAcore instrument) have provided a valuable experimental approach to determining such constants. The standard method is based on measuring amounts of analyte bound at equilibrium for different analyte concentrations. During the course of a typical surface plasmon resonance experiment the measured equilibrium levels for a given analyte concentration often decrease. This appears to be due to a loss of activity of the protein coupled to the sensor chip or other phenomena. The loss in signal can lead to an erroneous determination of the equilibrium constant. A data analysis approach is introduced that aims to compensate for the loss of activity so that its influence on the results of the experiments is reduced.

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