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Compartmentation of inhibitors of proteinases A and B and carboxypeptidase Y in yeast

โœ Scribed by Heidrun Matern; Heinrich Betz; Helmut Holzer


Book ID
118309357
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
365 KB
Volume
60
Category
Article
ISSN
0006-291X

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Carboxypeptidase Y (CPY) is a gly~osylated yeast vacuolar protease used commercially for synthesis of peptides. To increase the production of CPY i n Saccharomyces cerevisiae we have placed its coding region (PRC1) under control of the strongly regulated yeast G A L l promoter on multicopy plasmids