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Comparison of two treatment methods on the purification of shrimp polyphenol oxidase

✍ Scribed by Chen, J S; Charest, D J; Marshall, M R; Wei, C I

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
324 KB
Volume
75
Category
Article
ISSN
0022-5142

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✦ Synopsis

The e †ects of di †erent stirring time and two treatment methods (salt and organic solvent) on the recovery of shrimp polyphenol oxidase (PPO) were investigated. Stirring for 30 min yielded maximal PPO recovery. With respect to PPO speciÐc activity, yield and puriÐcation fold enhancement, the use of butanol treatment followed by Phenyl Sepharose CL-4B chromatography was shown to be better than ammonium sulphate fractionation and then Phenyl Sepharose chromatography. White shrimp PPO was more susceptible than pink shrimp PPO to inactivation during puriÐcation.

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