𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Comparison of the properties of the alcohol dehydrogenases from wild-type and mutant Chinese hamster somatic cells

✍ Scribed by B. G. Talbot; J. P. Thirion

Publisher
Springer
Year
1979
Tongue
English
Weight
663 KB
Volume
17
Category
Article
ISSN
0006-2928

No coin nor oath required. For personal study only.

✦ Synopsis

Alcohol dehydrogenases (alcohol: NAD oxidoreductase, E.C. 1.1.1.1.) from allyl alcohol-resistant and wild-type Chinese hamster cells were purified using gel filtration, ion-exchange, and affinity-column chromatography. Both enzymes exhibited the same isozyme band patterns on electrophoresis and isoelectric focusing. Physicochemical properties of the two enzymes such as pH and temperature optima, Km values, and temperature stability were found to be the same within the experimental errors. The genetic significance of these findings is discussed.

πŸ“œ SIMILAR VOLUMES

The kinetics and feedback inhibition of
The kinetics and feedback inhibition of cytidine 5β€²-triphosphate synthetase in wild-type and mutant Chinese hamster cells
✍ John D. McLaren; Ernest H. Y. Chu πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 512 KB

The kinetics and cytidine 5'-triphosphate (CTP) feedback inhibition of CTP synthetase in wild-type and four mutants of Chinese hamster V79 cells have been studied. The enzymes of the wild type and three of the four mutants exhibited positive cooperativity with the substrate uridine 5'-triphosphate (

Action of FdUrd and dCyd on the incorpor
Action of FdUrd and dCyd on the incorporation of brdurd in Chinese hamster somatic cell DNA and the isolation of auxotrophic mutants
✍ Romain Labrecque; Jean-Paul Thirion πŸ“‚ Article πŸ“… 1977 πŸ› John Wiley and Sons 🌐 English βš– 435 KB

## Abstract Chinese hamster somatic cells grown in the presence of bromodeoxyuridine, deoxycytidine and fluorodeoxyuridine incorporate more bromodeoxyuridine in their DNA than cells grown in the presence of bromodeoxyuridine alone. Thus they become more sensitive to light irradiation. Our data sugg

Sequence of the mglB gene from Escherich
Sequence of the mglB gene from Escherichia coli K12: Comparison of wild-type and mutant galactose chemoreceptors
✍ Scholle, Annette ;Vreemann, JΓΆrg ;Blank, Volker ;Nold, Annette ;Boos, Winfried ; πŸ“‚ Article πŸ“… 1987 πŸ› Springer 🌐 English βš– 866 KB

The mglB gene of Escherichia coli codes for a galactose-binding protein (GBP) that serves both as the galactose chemoreceptor and as the recognition component of the beta-methylgalactoside transport system. The mglB551 mutation eliminates the chemotactic function of GBP without altering its transpor

Quantitative immunofluorescence and immu
Quantitative immunofluorescence and immunoelectron microscopy of the topoisomerase IIΞ± associated with nuclear matrices from wild-type and drug-resistant Chinese hamster ovary cell lines
✍ Nikola I. Valkov; Jana L. Gump; Daniel M. Sullivan πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 908 KB

Topo II alpha is considered an important constituent of the nuclear matrix, serving as a fastener of DNA loops to the underlying filamentous scaffolding network. To further define a mechanism of drug resistance to topo II poisons, we studied the quantity of topo II alpha associated with the nuclear

Molecular organization of the outer memb
Molecular organization of the outer membrane ofSalmonella typhimuriumdifferent release of lipopolysaccharide from wild type and lipopolysaccharide mutant cells by EDTA treatment
✍ Jobst Gmeiner; Hildegard Bergmann; Siegfried Schlecht πŸ“‚ Article πŸ“… 1980 πŸ› Springer 🌐 English βš– 287 KB

Cells of Salmonella typhimurium wild type and o f several well defined lipopolysaccharide mutants were treated with E D T A . The percentage release of lipopolysaccharide and p h o s p h o l i p i d was determined. The results obtained show that the release o f l i p o p o l ysaccharide by E D T A d