Molecular organization of the outer membrane ofSalmonella typhimuriumdifferent release of lipopolysaccharide from wild type and lipopolysaccharide mutant cells by EDTA treatment
✍ Scribed by Jobst Gmeiner; Hildegard Bergmann; Siegfried Schlecht
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 287 KB
- Volume
- 124
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
Cells of Salmonella typhimurium wild type and o f several well defined lipopolysaccharide mutants were treated with E D T A . The percentage release of lipopolysaccharide and p h o s p h o l i p i d was determined. The results obtained show that the release o f l i p o p o l ysaccharide by E D T A declines along with the gradually diminishing chain length of the lipopolysaccharide, although the total a m o u n t of lipopolysaccharide was found to increase at the same time in the respective mutants. Implications of these findings for the organization of the outer m e m b r a n e are discussed.