Comparison of the killer toxin of several yeasts and the purification of a toxin of type K2

✍ Scribed by P. Pfeiffer; F. Radler

• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 543 KB
• Volume: 137
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00410734

✦ Synopsis

A total of 13 killer toxin producing strains belonging to the genera Saceharomyees, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saceharomyees cerevisiae, 2 different toxins of Pichia and one toxin of Candida were recognized. The culture filtrate of Pichia and Candida showed a much smaller activity than the strains of Saccharomyces. Extracellular killer toxins of 3 types of Saccharomyees were concentrated and partially purified. The pH optimum and the isoelectric point were determined. The killer toxins of S. eerevisiae strain NCYC 738, strain 399 and strain 28 were glycoproteins and had a molecular weight of Mr = 16,000. The amino acid composition of the toxin type K2 of S. cerevisiae strain 399 was determined and compared with the composition of two other toxins.