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Comparison of the enzymatic activity of commercial and semipurified lipase of Candida cylindracea in the hydrolysis of the esters of (R,S) 2-aryl propionic acids.

✍ Scribed by María J. Hernáiz; José M. Sanchez-Montero; José V. Sinisterra

Publisher
Elsevier Science
Year
1994
Tongue
French
Weight
664 KB
Volume
50
Category
Article
ISSN
0040-4020

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✦ Synopsis

A semipuriied lipaw of Candida cylindmdea (2.S) -easily obtainedfrom commercial crude lipase (LC)-is used in rhe enantiosekctive hydrolysis of (R,S) 2-arylpropionates. Ihe semipurification treatmen diminishu the lipase adwhy more than the &erase activity. i'he aaiiition of lactose (24 h) increases both activitks. LS is more active than LC -at the same amount of protein-in the hydrolysis of (R,S) Z-a19 propionates. lhis semipuriJcation showed a remarkabk bnprovement in yieki in the enamioespecific hydrolysis of these esters.

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