𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Comparison of Spectrophotometric, Fluorometric and High Performance Liquid Chromatography Methods for Determination of Chlorophyll a in Aquatic Samples: Effects of Solvent and Extraction Procedures

✍ Scribed by Dr. Timothy R. Jacobsen; Dr. Hakumat Rai

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
753 KB
Volume
75
Category
Article
ISSN
1434-2944

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✦ Synopsis

Chlorophyll u detcmiinations were made on lakewatcr a.nd algal samples by spectrophotometric, fluorometric and high performance liquid chromatographic methods. Acetone, methanol and etha-no1 solvents were evnluated for their ability to extract photosynthetic pigments from Scenedesrrius sp. cultures. Routinely used methods overestimated the chlorophyll a, concentrations present in the samples. Significant diffcrrnrcs resulted when various standard equations were used to calcu1at.e chlorophylf a concentrations. Acetone did not qumtit.;i,tively cxt,ract. chlorophyll pigmcnts, rvcn after 24 h. Mechanical disruption was found to hc important in assuring complete extrwtion of the chlorophyll pigments. samples containing degradation products.-Limnol. Oceanogr. 84: 183-186.

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