Comparison of two extraction procedures for determination of drugs of abuse in human saliva by high-performance liquid chromatography

✍ Scribed by P. Fernández; L. Morales; C. Vázquez; M. Lago; A. M. Bermejo

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 189 KB
• Volume: 28
• Category: Article
• ISSN: 0260-437X
• DOI: 10.1002/jat.1365

✦ Synopsis

Abstract

High performance liquid chromatography in combination with diode array detection (HPLC‐DAD) was used to determine morphine, 6‐acetylmorphine, cocaine, benzoylecgonine, cocaethylene, methadone and 2‐ethylene‐1,5‐dimethyl‐3,3,‐diphenylpyrrolidine in human saliva. For comparison, samples were prepared by either liquid–liquid extraction in Toxitubes A^®^ or microwave‐assisted extraction (MAE), by mixing 1 ml of saliva with 10 ml of chloroform and operating at 100 ˚C for 10 min. Acetonitrile and 0.02 m phosphate buffer at pH 6.5 were used as mobile phase in HPLC in gradient mode. The detector response was linear over the drug concentration range of 0.05–2.0 µg ml^−1^ in human saliva. The analytical method was validated by determining its precision and accuracy (n = 5), which were lower than 5% as relative standard deviation and 6% as relative error. Limits of detection ranged from 10 to 35 ng ml^−1^; mean recoveries of drugs were from 53 to 95% with Toxitubes A and from 83 to 100% with MAE at two different concentrations (0.1 and 1.0 µg ml^−1^). The proposed method was applied to 24 saliva samples from individuals poisoned with opiates and/or cocaine. Copyright © 2008 John Wiley & Sons, Ltd.