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Comparison of serum hydroquinidine determination by fluorescence polarization immunoassay and liquid chromatography

✍ Scribed by M. Tod; G. Resplandy; R. Farinotti; Y. Provost; A. Dauphin

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
363 KB
Volume
8
Category
Article
ISSN
0731-7085

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✦ Synopsis

Hydroquinidine is a structural analogue of quinidine. It is used in the treatment and prevention of cardiac arrhythmias and necessitates serum monitoring. Fluorescence polarization immunoassay (FPIA) of quinidine has been proposed and we have tested the performance of this assay for hydroquinidine using its cross-reaction with quinidine. Tracer (quinidine labelled with fluorescein) and anti-serum were purchased from Abbott S.A. Standard curves were obtained using specifically prepared hydroquinidine calibrators and within-run and run-to-run precision values (expressed as relative standard deviation) (RSD) lower than 5.3% (n = 10). In order to evaluate specificity of this assay in the clinical situation, FPIA and liquid chromatography results were compared.

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