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Comparison of ribosomal proteins of chloroplast from Spinach and of E. coli

✍ Scribed by Dorne, A. M. ;Eneas-Filho, J. ;Heizmann, P. ;Mache, R.

Publisher
Springer
Year
1984
Tongue
English
Weight
632 KB
Volume
193
Category
Article
ISSN
0026-8925

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✦ Synopsis

A comparison of ribosomal proteins from Escherichia coli and from chloroplasts of Spinach was made using two separate methods: electrophoretic migration and immunochemical cross-reaction between blotted E. coli ribosomal proteins and chloroplast ribosomal subunits antisera. It is shown that L2 from E. coli (E-L2) and L4 from chloroplasts (CS-L4) comigrated and that E-L2 immunologically cross-reacted with the isolated CS-L4 antibody. Co-migration was observed for three additional couples of 50S ribosomal proteins. It is also shown that at least one 30S E. coli ribosomal protein immuno-cross reacted with a 30S chloroplast antiserum and that three couples of 30S ribosomal proteins comigrated.

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