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Identification of anEscherichia coliS1-like protein in the spinach chloroplast ribosome

✍ Scribed by Véronique Hahn; Anne-Marie Dorne; Régis Mache; Jean-Pierre Ebel; Patrick Stiegler

Publisher: Springer
Year: 1988
Tongue: English
Weight: 890 KB
Volume: 10
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00014951

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✦ Synopsis

Antibodies directed against E. coli ribosomal protein S1 were used in immunoblotting assays to search for an S1-like protein in the ribosome of spinach chloroplast. An immunological cross-reaction was reproducibly detected on the blots and inhibition experiments have demonstrated its specificity. The chloroplastic ribosomal protein which has epitopes common to antigenic determinants of the E. coli protein S1 was identified as being protein S2/S3.

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