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Comparison of preparative reversed phase liquid chromatography and countercurrent chromatography for the kilogram scale purification of crude spinetoram insecticide

✍ Scribed by Carl DeAmicis; Neil A. Edwards; Michael B. Giles; Guy H. Harris; Peter Hewitson; Lee Janaway; Svetlana Ignatova

Book ID: 113535979
Publisher: Elsevier Science
Year: 2011
Tongue: English
Weight: 411 KB
Volume: 1218
Category: Article
ISSN: 1873-3778
DOI: 10.1016/j.chroma.2011.06.073

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## Abstract A systematic optimization of the HPLC separation of a mixture containing 11 pollutant phenols (PPs) using a Hypersil ODS (250 mm×4.6 mm, 5 μm) column and UV‐DAD detection has been carried out. The binary mobile phases used were obtained by mixing 50 mM phosphate (pH = 3.0) and methanol,