Comparison of the performance of different reversed-phase columns for liquid chromatography separation of 11 pollutant phenols

✍ Scribed by M. Cledera-Castro; A. Santos-Montes; R. Izquierdo-Hornillos; R. Gonzalo-Lumbreras

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 595 KB
• Volume: 30
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200600301

✦ Synopsis

Abstract

A systematic optimization of the HPLC separation of a mixture containing 11 pollutant phenols (PPs) using a Hypersil ODS (250 mm×4.6 mm, 5 μm) column and UV‐DAD detection has been carried out. The binary mobile phases used were obtained by mixing 50 mM phosphate (pH = 3.0) and methanol, ACN, or THF as organic modifiers. After selecting ACN as an organic modifier, the effects of pH and temperature on PPs separation were studied. A mobile phase of 50 mM acetate (pH = 5.0)–ACN (60 : 40 v/v) at 50°C allowed the separation of 11 phenols but not to baseline in 17 min. To improve the performance of this separation, the following RP columns were tested: Luna C18 (2), Purospher C18, Synergi C12, Synergi Fusion C18, Gemini C18, Luna Cyano, Lichrospher C8, and Envirosep‐PP (polymeric). In all the cases, the performance (analysis time, retention, selectivity, resolution, asymmetry factors, and efficiency) was evaluated. A further reoptimization of the mobile phase was carried out for all the columns by studying the ACN content and pH, with the aim of improving the above‐mentioned separations and selecting the most suitable one for PPs analysis.