Comparison of polypyrrole-based xanthine oxidase amperometric and potentiometric biosensors for hypoxanthine
โ Scribed by A.T. Lawal; S.B. Adeloju
- Book ID
- 113804695
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 222 KB
- Volume
- 66
- Category
- Article
- ISSN
- 1381-1177
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๐ SIMILAR VOLUMES
Fabrication and characterization of miniaturized amperometric hypoxanthine biosensors are described and demonstrated for monitoring hypoxanthine in myocardial cell culture media. The sensors are based on xanthine oxidase (XO) immobilized on carbon fiber microelectrodes (CFMEs) using a composite film
Deflavo xanthine oxidase was prepared by removing the flavin adenine dinucleotide (FAD) group from the native enzyme using 2 M CaCl,. Unlike the native enzyme, the resulting deflavo xanthine oxidase no longer reacted with dioxygen for the oxidation of hypoxanthine. However, its capability for oxidiz