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Characterization of a mediated amperometric biosensor for hypoxanthine using deflavo xanthine oxidase

โœ Scribed by Shishan Zhao; John H. T. Luong


Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
900 KB
Volume
6
Category
Article
ISSN
1040-0397

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Deflavo xanthine oxidase was prepared by removing the flavin adenine dinucleotide (FAD) group from the native enzyme using 2 M CaCl,. Unlike the native enzyme, the resulting deflavo xanthine oxidase no longer reacted with dioxygen for the oxidation of hypoxanthine. However, its capability for oxidiz

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A mediated amperometric sensor for measuring rglutamate is described. The sensor is based on a carbon paste electrode containing the electron transfer mediator tetrathiafulvalene (TTF), and the enzyme L-glutamate oxidase immobilixed on the electrode surface. The enzyme is crosslinked with glutaralde