Deflavo xanthine oxidase was prepared by removing the flavin adenine dinucleotide (FAD) group from the native enzyme using 2 M CaCl,. Unlike the native enzyme, the resulting deflavo xanthine oxidase no longer reacted with dioxygen for the oxidation of hypoxanthine. However, its capability for oxidiz
โฆ LIBER โฆ
Characterization of a mediated amperometric biosensor for hypoxanthine using deflavo xanthine oxidase
โ Scribed by Shishan Zhao; John H. T. Luong
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 900 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1040-0397
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A mediated amperometric sensor for measuring rglutamate is described. The sensor is based on a carbon paste electrode containing the electron transfer mediator tetrathiafulvalene (TTF), and the enzyme L-glutamate oxidase immobilixed on the electrode surface. The enzyme is crosslinked with glutaralde