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Comparison of MTT and ATP-based assays for the measurement of viable cell number

✍ Scribed by Petty, Russell D. ;Sutherland, Lesley A. ;Hunter, Elizabeth M. ;Cree, Ian A.

Publisher: John Wiley and Sons
Year: 1995
Weight: 458 KB
Volume: 10
Category: Article
ISSN: 0884-3996
DOI: 10.1002/bio.1170100105

No coin nor oath required. For personal study only.

✦ Synopsis

Cell viability assays are widely used t o assess the effect of chemotherapeutic drugs and other agents on cell lines and have shown promise for the prediction of tumour chemosensitivity. In this study w e have compared t w o viability assays using Daudi and CCRF-CEM cell lines over a range of 1500-100,000 cells/well of a microplate. The ATP assay was able t o detect the lower limit of 1563 cells/well w i t h luminescence values at least 1OOx background readings, while the MTT assay could not detect less than 25,000 cells/ well above background readings. The ATP assay also showed better reproducibility and sensitivity when cells were grown in microtitre plates over several days, and is particularly useful for the measurement of viability w i t h low cell numbers.

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