A novel dielectrophoresis-based device for the selective retention of viable cells in cell culture media

✍ Scribed by Aristides Docoslis; Nicolas Kalogerakis; Leo A. Behie; Karan V. I. S. Kaler

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 490 KB
• Volume: 54
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19970505)54:3<239::aid-bit5>3.0.co;2-g

✦ Synopsis

Cost-effective production of biopharmaceuti-scale production, perfusion cultures represent the most cals on a large scale can be carried out by perfusion cost-effective mode of operation. Perfusion cultures are cultures of mammalian cells. One problem with this mode characterized by the continuous replenishment of the of operation for submerged free-cell cultures is the reculture medium with fresh medium and the removal of quirement for an efficient cell separation device located toxins and byproducts with the effluent stream. The key in the effluent stream. The present work investigates characteristic of a perfusion culture is the retention of the potential for the development of a novel dielectrophoresis-based cell separator, capable of providing selecthe cells in the bioreactor, i.e., no cells are removed by tive retention of viable cells in cell culture media, which the effluent stream. Perfusion results in high cell densiare highly conductive. Predictions of the dielectrophoretic ties (10-fold or higher than those of typical batch cul-(DEP) response in culture media were first obtained

tures) (Al-Rubeai et al., 1992; Bu ¨ntemeyer et al., 1994; through a series of DEP-levitation experiments. Sub-