Comparison of monoclonal antibodies for flow cytometric analysis of HLA-B27 antigen
β Scribed by Anne M. Ward; Afzal Nikaein
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 358 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A biannual external quality assurance (EQA) scheme for flow cytometric typing of the HLA-B27 antigen is operational in The Netherlands and Belgium since 1995. We report here on the results of the first seven send-outs to which 36 to 47 laboratories participated. With the send-out, four specimens fro
Typing for HLA-B27 by serological methods is routinely performed using the microlymphocytotoxic test (MLCT). Since monoclonal antibodies (MAB) against HLA-B27 are available, flow cytometry (FC), which requires less time than the MLCT has been developed as an alternative technique. The aim of the pre
## Abstract ## Background Human leukocyte antigen B27 (HLAβB27) is a major histocompatibility complex class 1 molecule that is strongly associated with the disease ankylosing spondylitis. Testing for HLAβB27 is of diagnostic value because 90% of patients with ankylosing spondylitis have the B27 an
For evaluation of specificity and sensitivity of flowcytometric determination of HLA-B27 antigen, we determined the HLA-B27 on lymphocytes using HLA-B27 monoclonal antibody by flow cytometer. Data were compared to those by conventional Terasaki microlymphocytoxicity test and DNA genotyping Polymeras