Comparison of methods for assessing integrity of equine sperm membranes
β Scribed by M.L. Foster; C.C. Love; D.D. Varner; S.P. Brinsko; K. Hinrichs; S. Teague; K. LaCaze; T.L. Blanchard
- Book ID
- 116912497
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 705 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0093-691X
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Sperm DNA damage is common and has been associated with reduced rates of conception, impaired embryonic development and increased risk of miscarriage. Although the exact causes of sperm DNA damage are unknown, it is clear that infertile men possess substantially higher levels of sperm DNA damage tha
Eight different methods were used to assess the cell viability of four strains of Saccharomyces. Staining with Mg-ANS, primuline yellow, FITC and methylene blue gave a good index of yeast cell viability. The standard plate count technique and microcolony formation also gave a good measure of cell vi