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A comparison of methods for assessing yeast viability

✍ Scribed by J. T. Trevors; R. L. Merrick; I. Russell; G. G. Stewart

Publisher
Springer Netherlands
Year
1983
Tongue
English
Weight
252 KB
Volume
5
Category
Article
ISSN
0141-5492

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✦ Synopsis

Eight different methods were used to assess the cell viability of four strains of Saccharomyces. Staining with Mg-ANS, primuline yellow, FITC and methylene blue gave a good index of yeast cell viability. The standard plate count technique and microcolony formation also gave a good measure of cell viability. Fluorescent staining with acridine orange was the least useful of the methods tested, INT dye reduction gave a good index of respiring cells depending upon the yeast strain tested.

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