✦ LIBER ✦

Comparison of in Vitro Transcriptions Using Various Types of DNA Templates

✍ Scribed by V. Kohli; J. Temsamani

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 408 KB
Volume: 208
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1993.1036

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✦ Synopsis

Improvements in the synthesis of RNA using T7 RNA polymerase and synthetic deoxyoligonucleotides containing the T7 RNA promoter are described. Yields were highest when the double-stranded promoter was generated from a hairpin hybridized to the singlestranded DNA template or when the templates were assembled by ligation of shorter chemically synthesized deoxyoligonucleotides. Our results show that for the synthesis of RNA, templates prepared by ligating smaller deoxyoligonucleotides were as much as 25 times more efficient than those prepared by one-step chemical synthesis. ic 1983 Academic Press, inc.

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