In vitro transcription assay of ozonated T7 phage dna
โ Scribed by Catherine Mura; Young Sup Chung; E. R. Nestman
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 327 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0893-6692
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๐ SIMILAR VOLUMES
E coli RNA polymerase holoenzyme is able to recognize transcription initiation sites on Adenovirus 2 DNA that are functionally indistinguishable from promoters for the enzyme on phage DNAs. The complexes formed between the polymerase and the DNA at these sites can exist in two states-either as I (in
We estimate that E. coli RNA polymerase is able to form stable, rifampicin-resistant, pre-intiation complexes with Adenovirus 2 DNA at three to six binding sites. The number of RNA chains initiated from such complexes has been determined form the incorporation of gamma-32P-ATP and -GTP at two rifamp