Comparison of Human Serum Dopamine-β -Hydroxylase Levels by Radioimmunoassay and Enzymatic Assay
✍ Scribed by Robert A. Rush, Paul E. Thomas, Toshiharu Nagatsu and Sidney Udenfriend
- Book ID
- 123652573
- Publisher
- National Academy of Sciences
- Year
- 1974
- Tongue
- English
- Weight
- 448 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/63746
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📜 SIMILAR VOLUMES
1. Two different molecular forms of dopamine-beta-hydroxylase were isolated from human serum; a major component (Peak I enzyme) with a molecular weight of 368000 and with a higher specific activity and a minor component (Peak II enzyme) with a molecular weight of 188000 and with a lower specific act
As a model for the assay of enzyme activity by high-performance liquid chromatography, a simple, sensitive and specific fluorescence assay for dopamine P-hydroxylase (DBH) activity was devised using o-phthalaldehyde as reagent. Tyramine was used as a substrate and was incubated under optimal conditi