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Comparison of flow cytometric methods for the measurement of ZAP-70 expression in a routine diagnostic laboratory

โœ Scribed by G. GIBBS; T. BROMIDGE; D. HOWE; J. HOPKINS; S. JOHNSON


Book ID
108701699
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
190 KB
Volume
27
Category
Article
ISSN
1751-5521

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โœ Mark Shenkin; Russell Maiese ๐Ÿ“‚ Article ๐Ÿ“… 2006 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 747 KB

## Abstract ## Background: In this study we developed a method to measure the amount of ZAPโ€70 [zeta accessory protein] in Bโ€CLL cells without relying on the ZAPโ€70 expression of patient B or T cells to normalize fluorescence intensity. ## Methods: Bโ€CLL cells were fixed with formaldehyde before