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Comparison of different staining methods for polyvinylidene difluoride membranes

✍ Scribed by Jesper Christiansen; Dr. Gunnar Houen

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
520 KB
Volume
13
Category
Article
ISSN
0173-0835

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✦ Synopsis

Compariron of different staining methods for PVDF membranes

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Method for rapid electrophoretic transfe
Method for rapid electrophoretic transfer of isoelectric focusing gels to polyvinylidene difluoride
✍ Dr. Sharon Lobert; John J. Correia πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 199 KB

## Abstract We present here a protocol for unidirectional wet electrotransfer of isoelectric focusing (IEF) gels to polyvinylidene difluoride (PVDF) that can be carried out in < 2 h, including preequilibration steps. In developing this protocol, we systematically evaluated sodium dodecyl sulfate an

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Methods have been developed for high-sensitivity sequence analysis of proteins electroblotted onto polyvinylidene difluoride (PVDF) membranes using a Hewlett-Packard G1005A protein sequencer. This sequencer normally uses a biphasic (hydrophobic/hydrophilic) reaction column which was designed to acco