Comparison of different capillary electrophoresis methods for analysis of recombinant erythropoietin glycoforms
✍ Scribed by Pilar López-Soto-Yarritu; Jose Carlos Díez-Masa; Mercedes de Frutos; Alejandro Cifuentes
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 114 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1615-9306
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## Abstract For Abstract see ChemInform Abstract in Full Text.
Two methods for the collection of capillary electrophoresis fractions for analysis by matrix-assisted laser desorption/ionization have been investigated; the first using a novel ion-porous joint design for dropwise fraction collection and the second, a process of electrospray deposition. Sensitivity
Two different capillary electrophoretic separation modes for the analysis of UDP-sugars have been tested. In comparison to the traditionally used HPLC method the micellar electrokinetic capillary chromatography (MECC) as well as the capillary zone electrophoresis (CZE) results in an improvement of t