Comparison of derivatization and chromatographic methods for GC–MS analysis of amino acid enantiomers in physiological samples
✍ Scribed by Magdalena C. Waldhier; Katja Dettmer; Michael A. Gruber; Peter J. Oefner
- Book ID
- 108161155
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 681 KB
- Volume
- 878
- Category
- Article
- ISSN
- 1873-376X
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The amounts and the configuration of the stereoisomers of 2,6-diaminopimelic acid (Dap) and the enantiomeric content of other amino acids were determined in five individual species (Fibrobacter succinogenes, Streptococcus bovis, Selenomonas ruminantium, Prevotella ruminicola and Anaerovibrio lipolyt
## Abstract Three derivatization agents used in GC analysis of amino acids were compared: __N,O__‐bis(trimethylsilyl)trifluoroacetamide, (BSTFA), __N__‐methyl‐__N__‐(__tert__‐butyldimethylsilyl)trifluoroacetamide (MTBSTFA), and isobutyl chloroformate (iBuCF). It was shown that the analytical charac