Comparison of CEA and CA549 in human breast tumour tissue extracts

✍ Scribed by E.D. Ryan; G. Bilous

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 111 KB
• Volume: 27
• Category: Article
• ISSN: 0009-9120
• DOI: 10.1016/0009-9120(94)90064-7

✦ Synopsis

CHEMISTS

diagnosis difficult on a clinical basis alone. Direct DNA genotyping at the FMR-1 locus has replaced classical cytogenetic testing for confirming the diagnosis as well as for carrier identification. DNA analysis methods are cumbersome and not suited for analysis of the FMR-I locus in all clinical situations where the syndrome is suspected. Recently, analysis of the fragile X protein was made possible by the production of antibodies to the gene product: pFMR-1. The antibodies detect a few protein bands in leukocytes of normal individuals and in transfected cell lines whereas in affected individuals, the FMR-1 protein is absent. Using a monoclonal antibody against pFMR-1, we tested its presence in human serum and plasma with the aim of developping an automated serologic test for diagnosis of fragile X syndrome. Using Western blotting, no FMR-l protein was detected in 13 normal human serum, 3 plasma and 3 whole blood samples. However, when leukocytes were isolated and tested separately, pFMR-l was detected. These results suggests that pFMR-1 is not present in whole blood, serum or plasma in a sufficient quantity in normal individuals to be detected by standard immunoassays.