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Comparison of bovine and porcine β-lactoglobulin: a mass spectrometric analysis

✍ Scribed by Gaetano Invernizzi; Maria Šamalikova; Stefania Brocca; Marina Lotti; Henriette Molinari; Rita Grandori

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
873 KB
Volume
41
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

Nano‐electrospray‐ionization mass spectrometry (nano‐ESI‐MS) is applied to comparison of bovine and porcine β‐lactoglobulin (BLG and PLG). The conformational and oligomeric properties of the two proteins under different solvent and experimental conditions are analyzed. The pH‐dependence of dimerization is described for the pH range 2–11. The results indicate maximal dimer accumulation at pH 6 for BLG and pH 4 for PLG, as well as a lower stability of the PLG dimer at pH 4 compared to BLG at pH 6. Conformational stability appears to be higher for BLG at acidic pH, but higher for PLG at basic pH. The higher stability of BLG at low pH is revealed by means of either chemical or thermal denaturation. Equilibrium folding intermediates of both proteins are detected. Finally, conditions are found that promote dissociation of the BLG dimer at pH 6 into folded monomers. Copyright © 2006 John Wiley & Sons, Ltd.

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