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Comparison of an electrochemiluminescent homogenous immunoassay and an ELISA for monitoring productivity in mammalian cell bioreactors

โœ Scribed by Per I. Lindell; Lee Hall; Tai-Guang Wu; Charles L. Cooney


Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
325 KB
Volume
5
Category
Article
ISSN
0951-208X

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โœฆ Synopsis


An Enzyme Linked Immuno Sorbent Assays (ELISA) and an Electro-chemiluminescent Immuno Assay (CIA) are compared for the purpose of monitoring product formation in mammalian cell bioreactors. The ELISA had a relative standard deviation of 10%, compared to 8% for the CIA. The CIA was found to be a fast and accurate alternative to the ELISA. The use of more than one immuno assay format was also shown to provide additional insight into process performance.


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