✦ LIBER ✦

Comparison of an electrochemiluminescent homogenous immunoassay and an ELISA for monitoring productivity in mammalian cell bioreactors

✍ Scribed by Per I. Lindell; Lee Hall; Tai-Guang Wu; Charles L. Cooney

Publisher: Springer-Verlag
Year: 1991
Tongue: English
Weight: 325 KB
Volume: 5
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00152779

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✦ Synopsis

An Enzyme Linked Immuno Sorbent Assays (ELISA) and an Electro-chemiluminescent Immuno Assay (CIA) are compared for the purpose of monitoring product formation in mammalian cell bioreactors. The ELISA had a relative standard deviation of 10%, compared to 8% for the CIA. The CIA was found to be a fast and accurate alternative to the ELISA. The use of more than one immuno assay format was also shown to provide additional insight into process performance.

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