Nylon microfiltration membranes were coated with dextrans (M r โ 6000 and 40,000) and poly(vinylalcohol) (PVA) (M r โ72,000) to produce affinity matrices with adequate chemical functionality and low non-specific protein binding characteristics. In all cases, a considerable reduction of non-specific
โฆ LIBER โฆ
Comparison of affinity membranes and conventional affinity matrices with regard to protein purification
โ Scribed by S. Krause; K. H. Kroner; W. D. Deckwer
- Publisher
- Springer-Verlag
- Year
- 1991
- Tongue
- English
- Weight
- 266 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0951-208X
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Alkyl-Sepharose 4B with octyl, decyl, or dodecyl groups as an alkyl chain was a good adsorbent for any type of detergents and a variety of proteins, but not for phospholipids in a vesicle form. When these gels were added to the mixtures of reconstituted proteoliposomes prepared by using bovine band