Comparison of acyl-carrier protein and other protein structures in aqueous solutions by Fourier-transform infrared spectroscopy
โ Scribed by Mary Lou Ernst-Fonberg; Lesa M.S. Worsham; Sande G. Williams
- Book ID
- 113269205
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 877 KB
- Volume
- 1164
- Category
- Article
- ISSN
- 0167-4838
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๐ SIMILAR VOLUMES
An approach to the determination of secondary structure content in proteins in aqueous solutions based on attenuated total reflection (ATR) Fourier transform infrared (FT-IR) spectrometry is proposed. ATR-FT-IR spectra of eleven proteins with known crystal structures were recorded. An algorithm for
FT-IR/PAS (Fourier transform infrared/photoacoustic spectroscopy) was used to evaluate the secondary structure of proteins. Four well-studied proteins, concanavalin A, hemoglobin, lysozyme, and trypsin, which have different distributions of secondary structures, were used for assignments of the infr