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Comparison of a modified spectrophotometric and the pH-stat methods for determination of the degree of hydrolysis of whey proteins hydrolysed in a tangential-flow filter membrane reactor

✍ Scribed by Seronei Chelulei Cheison; Shao-Bing Zhang; Zhang Wang; Shi-Ying Xu

Book ID
116488415
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
472 KB
Volume
42
Category
Article
ISSN
0963-9969

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Hydrolysis of whey protein isolate in a
Hydrolysis of whey protein isolate in a tangential flow filter membrane reactor: I. Characterisation of permeate flux and product recovery by multivariate data analysis
✍ Seronei Chelulei Cheison; Zhang Wang; Shi-Ying Xu πŸ“‚ Article πŸ“… 2006 πŸ› Elsevier Science 🌐 English βš– 466 KB

Protease N (IUB 3.4.24.28, Bacillus subtilis) enzyme was used to continuously hydrolyse an initial 5% (w/v) whey protein isolate (86.98%, Kjeldahl nitrogen Γ— 6.38) for 5 h at pH 7.0 and 55 β€’ C in a 10 kDa tangential flow filter (TFF) enzymatic membrane reactor (EMR). The retentate temperature (A: 25