Hydrolysis of whey protein isolate in a tangential flow filter membrane reactor: II. Characterisation for the fate of the enzyme by multivariate data analysis

Hydrolysis of whey protein isolate in a

Hydrolysis of whey protein isolate in a tangential flow filter membrane reactor: I. Characterisation of permeate flux and product recovery by multivariate data analysis

✍ Seronei Chelulei Cheison; Zhang Wang; Shi-Ying Xu 📂 Article 📅 2006 🏛 Elsevier Science 🌐 English ⚖ 466 KB

Protease N (IUB 3.4.24.28, Bacillus subtilis) enzyme was used to continuously hydrolyse an initial 5% (w/v) whey protein isolate (86.98%, Kjeldahl nitrogen × 6.38) for 5 h at pH 7.0 and 55 • C in a 10 kDa tangential flow filter (TFF) enzymatic membrane reactor (EMR). The retentate temperature (A: 25