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Comparison between flow cytometry and image cytometry in ploidy distribution assessments in gynecologic cancer

✍ Scribed by Dr. Janne Kaern; Jarle Wetteland; Claes G. Tropé; George W. Farrants; Sang-Woo Juhng; Erik O. Pettersen; Albrecht Reith; Håvard E. Danielsen

Book ID
102818189
Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
647 KB
Volume
13
Category
Article
ISSN
0196-4763

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✦ Synopsis

The DNA content in 37 tumors from 34 women with gynecological cancer was measured by flow cytometry (FCM) and interactive image cytometry (ICM). Agreement was obtained in 81% of cases as regards ploidy levels, but seven tumors (19%) showed different ploidies. Of these, five were classified as diploid by FCM but either aneuploid (three cases) or polyploid (two cases) by ICM. Two other tumors were aneuploid by ICM but polyploid (one case) and unclassifiable (one case) by FCM. All tumors classified as aneuploid by FCM were also aneuploid by ICM, and all tumors classified diploid 'This study was supported by the Norwegian Cancer Society (grants 88234 and R8290).

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