Ki-67 labeling indices in non-small cell lung cancer: Comparison between image cytometry and flow cytometry
✍ Scribed by Akihiko Ishikuro; Hirotoshi Dosaka-Akita; Masakazu Kato; Michihiro Fujino; Hiroshi Isobe; Hiroshi Miyamoto; Kazuaki Inoue; Yoshikazu Kawakami
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 148 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0196-4763
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✦ Synopsis
Expression of the proliferation-associated nuclear antigen
Ki-67 was studied in 27 human non-small cell lung cancers (NSCLCs). We measured immunohistochemical Ki-67 labeling indices using image cytometry (ICM) and flow cytometry (FCM) and compared the indices determined with the two methods. Ki-67 labeling indices of tumor cells ranged from 2% to 59% with ICM, and from 3% to 56% with FCM, varying from case to case. There was a linear correlation of values between the two methods (r 5 0.77, P F 0.05). To examine whether Ki-67 labeling indices represent tumor proliferative activity, we studied the relationship between the Ki-67 labeling index and the S-phase fraction determined by FCM. There was a positive correlation between the Ki-67 labeling index and the S-phase fraction (r 5 0.91, P F 0.01). Comparison of Ki-67 labeling indices and clinicopathological parameters showed lower Ki-67 labeling indices in well-differentiated tumors than in moderate and poorly differentiated tumors (P F 0.05). No correlation was observed between Ki-67 labeling indices and p53 expression. It was concluded that the Ki-67 labeling index was in fact measured with both ICM and FCM, and that it represents tumor proliferative activity of NSCLCs. Cytometry 30:186-191, 1997.