๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Comparative tumour localization properties of radiolabelled monoclonal antibody preparations of defined immunoreactivities

โœ Scribed by Malcolm V. Pimm; Robert W. Baldwin

Book ID
104683651
Publisher
Springer
Year
1987
Tongue
English
Weight
455 KB
Volume
13
Category
Article
ISSN
0340-6997

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โœฆ Synopsis

The immunoreactive fraction of an anti-CEA monoclonal antibody preparation has been progressively decreased by the addition of increasing proportions of impurity in the form of immunologically inert mouse immunoglobulin. Following radioiodination, the immunoreactive fractions of the preparations were determined and their localization in a human tumour xenograft in nude mice was assessed. There was a progressive decline in tumour localization, from tumour to blood ratios of 2:1 with unadulterated antibody to 0.6:1 with preparations only 15% with respect to the initial antibody. These findings demonstrate that the immunoreactive fraction of monoclonal antibody preparations is a major limiting factor in tumour localization and this has implications for experimental and clinical applications of monoclonal antibodies.

๐Ÿ“œ SIMILAR VOLUMES

Comparative biodistributions and rates o
Comparative biodistributions and rates of catabolism of radiolabelled anti-CEA monoclonal antibody and control immunoglobulin in nude mice with human tumour xenografts showing specific antibody localization
โœ M. V. Pimm; R. W. Baldwin ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Springer ๐ŸŒ English โš– 555 KB

The rate of catabolism of a radiolabelled monoclonal anti-CEA antibody has been compared with that of control IgG1 in control nude mice and in mice with CEA producing xenografts and antigen negative xenografts. The rate of catabolism of antibody, but not of IgG, was increased 3-4 fold in mice with x