Comparative Study of the Active Site Caging of Serine Proteases: Thrombin and Factor Xa †
✍ Scribed by Thuring, Jan Willem; Li, Hui; Porter, Ned A.
- Book ID
- 120508218
- Publisher
- American Chemical Society
- Year
- 2002
- Tongue
- English
- Weight
- 193 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0006-2960
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## Abstract Factor Xa (FXa) elicits intracellular signaling responses through the activation of protease‐activated receptor 2 (PAR2) and possibly also through PAR1 in endothelial cells. In this study, we investigated FXa signaling in endothelial cells when the protease was either in free form or as
The structure of two selective inhibitors, Ac-Tyr-Ile-Arg-Ile-Pro-NH 2 and Ac-(4-Amino-Phe)-(Cyclohexyl-Gly)-Arg-NH 2 , in the active site of the blood clotting enzyme factor Xa was determined by using transferred nuclear Overhauser effect nuclear magnetic resonance (NMR) spectroscopy. They represen