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Comparative study of solution phase RT-PCR and RT in situ PCR for HCV RNA in HCV infected patients

✍ Scribed by Zs. Simon; G. Lotz; B. Nemes; F. Szalav; G. Lengyel; M. Abonyi; E. Ibranyi; Zs. Schaff


Book ID
118568385
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
160 KB
Volume
28
Category
Article
ISSN
0168-8278

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A modified competitive RT-PCR (mcRT-PCR) to measure HCV RNA in serum and the Amplicor HCV Monitor assay were compared. For mcRT-PCR, the RNA extracted was retrotranscribed and coamplified in one step with a known amount of a DNA internal control (IC). Digoxigenin-labeled amplified products were hybr