Titration of intrahepatic (−) HCV RNA by strand-specific RT-PCR in chronic hepatitis C: morphological and clinical correlations
✍ Scribed by F. Negro; K. Krawczynski; R. Quadri; M. Mondelli; L. Rubbia-Brandt; M. Baud; J.-P. Zarski; A. Hadengue
- Book ID
- 118568364
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 164 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0168-8278
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Studies aimed at correlating the intrahepatic hepatitis C virus (HCV)-RNA level and anatomo-clinical features have been difficult because of sensitivity and specificity shortcomings of available techniques. We titered the genomic- and minus-strand HCV RNAs by a strand-specific, semiquantitative, gen
## Abstract HCV replicates in liver via an intermediate negative strand RNA. To study the relevance of HCV genome replication, quantitative strand‐specific HCV real‐time RT‐PCR assays were developed and applied to livers explanted because of end‐stage cirrhosis. The assays have broad ranges of dete