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Comparative study of six methods of protein extraction for two-dimensional gel electrophoresis of proteomic profiling in poplar stems

✍ Scribed by Azri, Wassim; Ennajah, Amel; Jing, Mai


Book ID
121230963
Publisher
Agricultural Institute of Canada
Year
2013
Tongue
English
Weight
729 KB
Volume
93
Category
Article
ISSN
0008-4220

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Two-dimensional gel electrophoresis of p
✍ Nasser Bahrman; Dominique Vienne; HervΓ© Thiellement; Jean-Paul Hofmann πŸ“‚ Article πŸ“… 1985 πŸ› Springer 🌐 English βš– 429 KB

A method of two-dimensional gel electrophoresis of proteins from Douglas fir needles is described. Extraction in the presence of sodium dodecyl sulfate (SDS) and 2-mercaptoethanol followed by heating at 100 degrees C produces reliable two-dimensional gels which are convenient for genetic studies. Th