𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Two-dimensional gel electrophoresis of proteins for genetic studies in douglas fir (Pseudotsuga menziesii)

✍ Scribed by Nasser Bahrman; Dominique Vienne; Hervé Thiellement; Jean-Paul Hofmann

Publisher
Springer
Year
1985
Tongue
English
Weight
429 KB
Volume
23
Category
Article
ISSN
0006-2928

No coin nor oath required. For personal study only.

✦ Synopsis

A method of two-dimensional gel electrophoresis of proteins from Douglas fir needles is described. Extraction in the presence of sodium dodecyl sulfate (SDS) and 2-mercaptoethanol followed by heating at 100 degrees C produces reliable two-dimensional gels which are convenient for genetic studies. Three genotypes from different geographical origins have been compared: among 225 loci expressed, 22 display regulatory variations and 7 show allelic variations. Thus it is now possible to undertake the genetic study of Douglas fir using this powerful technique.

📜 SIMILAR VOLUMES

In-gel digestion of proteins for interna
In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis
✍ Jorge Rosenfeld; Joël Capdevielle; Jean Claude Guillemot; Pascual Ferrara 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 940 KB

We examined the different steps necessary for the enzymatic digestion of proteins in the polyacrylamide matrix after gel electrophoresis. As a result, we developed an improved method for obtaining peptides for internal sequence analysis from 1-2 micrograms of in-gel-digested proteins. The long washi

Genetic polymorphism in Norway spruce,Pi
Genetic polymorphism in Norway spruce,Picea abies(L.) Karst, assessed by two-dimensional gel electrophoresis of needle proteins
✍ André Sieffert 📂 Article 📅 1988 🏛 Springer-Verlag 🌐 English ⚖ 955 KB

A comparative analysis of three Norway spruce genotypes by two-dimensional gel electrophoresis is presented. This study has led to the identification of approximately 970 gene products for each genotype. Significant qualitative and quantitative differences have been identified, and qualitative and q

Alterations of stress related proteins i
Alterations of stress related proteins in genetically altered mice revealed by two-dimensional differential in-gel electrophoresis analysis
✍ Heather A. Skynner; Thomas W. Rosahl; Michael R. Knowles; Kamran Salim; Lee Reid 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 254 KB 👁 2 views

Transgenic, knockout and knockin mice are useful tools for linking specific genes with behaviour and other complex biological processes. However, complications arising due to compensatory changes, genetic background differences and other factors could lead to difficulty in interpreting the resulting