✦ LIBER ✦

Comparative studies on the structural gene for the ribosomal protein S1 in ten bacterial species

✍ Scribed by Schnier, Joachim ;Faist, Gabriele

Book ID: 104770476
Publisher: Springer
Year: 1985
Tongue: English
Weight: 682 KB
Volume: 200
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00425734

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✦ Synopsis

By applying the Southern blot technique we compared the structural gene rpsA for ribosomal protein $I and its preceding sequence from Escherichia coIi with nine other bacterial species. We found high homology among the structural genes of E. coli and other gram-negative but not gram-positive bacteria. In contrast, the regulatory sequence preceding the structural gene was not highly conserved among the organisms studied.

Cloning and DNA sequence analysis of a 1.2 kb fragment coding for most of the structural gene for SI from Providencia localized some strongly conserved parts of the DNA sequence, despite the fact that the codon usage showed considerable divergence from that of E. coli.

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